Truncation analysis of the AmidP in cotyledons of transgenic Arabidopsis seedlings.

Abstract

<p>A. Promoter truncation, the primers binding sites and endonucleases sites are illustrated. The 1504 bp cloned sequence for the AmidP includes 4 nucleotides which are added to form an <i>Eco</i>RI acting site. All the fused structures are obtained by ligation of the pFGC-DR and the PCR products precut by <i>Eco</i>RI and <i>Nco</i>I respectively, except that of the P3-DR which is self-ligated with the P1-DR digested by <i>Bam</i>HI and <i>Bgl</i>II. B-G. GUS activities in the transgenic Arabidopsis cotyledons of P1-DR (B), P2-DR (C), P3-DR (D), P4-DR (E), P5-DR (F), and P6-DR (G).</p