The construction and results of the 5′- and 3′- deletions of <i>VVE</i> motif between −1500 and −1324 of the AmidP.

Abstract

<p>A. Sequence and element site analysis of the <i>VVE</i> motif in the AmidP. 4 nucleotides (grey box) are arbitrarily added to form an <i>Eco</i>RI acting site. Element sites for known transcription factors indicated as arrows are detected by AthaMap web tools (see “Materials and methods”). Vertical dotted line indicated deletion sites. B. Schematic diagram of the chimeric constructs. The numbers above the bars indicate the residual region of the <i>VVE</i> motif after 5′- or 3′- deletions. All the fused constructs are obtained by ligation of the pFGC-MiniGUS and the PCR products precut by <i>Eco</i>RI and <i>Bam</i>HI respectively. C–K. Representative histochemical stained cotyledon demonstrates the strength and specificity of GUS activities in the transgenic Arabidopsis of 5M1 (C), 5M2 (D), 5M3 (E), 5M4 (F), 3M1 (G), 3M2 (H), 3M3 (I), 3M4 (J), and 3M5 (K).</p