Phylogenetic tree, amino acid alignments, domains and modification sites of Spata33.

Abstract

<p>A). Phylogenetic tree of Spata33 in mammals. Phylogenetic analysis was performed with Phylip. Numbers on the branches represent the bootstrap values from 1000 replicates obtained using the Neighbor-Joining method. The scale bar corresponds to the estimated evolutionary distance units. GenBank accession numbers are as follows: <i>Callithrix jacchus</i>, XP_002761326.1; <i>Canis lupus familiaris</i>, XP_003434741.1; <i>Cricetulus griseus</i>, XP_003495126.1; <i>Gorilla gorilla gorilla</i>, XP_004058209.1; <i>Homo sapiens,</i> BAG64150.1; <i>Macaca mulatta,</i> XP_001104069.2; <i>Mus musculus</i>, NP_796253.2; <i>Nomascus leucogenys</i>, XP_003280677.1; <i>Otolemur garnettii,</i> XP_003800884.1; <i>Pan paniscus</i>, XP_003805830.1; <i>Pan troglodytes</i>, XP_511172.4; <i>Papio anubis,</i> P_003917381.1; <i>Rattus norvegicus</i>, NP_001099665.1; <i>Saimiri boliviensis boliviensis</i>, XP_003944616.1; <i>Tupaia_chinensis</i>, ELW62868.1. B). Alignment of amino acid sequences of the Spata33 proteins. Amino acids that are identical in all these species are shown in white letters on black background. The DUF4609 domain predicted by Pfam is boxed. C). Schematic mapping of potential protein domains and post-translational modification sties. The predicted sites for N-myristoylation, N-glycosylation and phosphorylations in Spata33 were indicated (N-Myr, N-myristoylation site; N-Glyc, N-glycosylation site; S/T, Serine/Threonine phosphorylation sites).</p