miR-142 downregulates MAOA protein expression in human neurons.

Abstract

<p>Human neurons grown in vitro for 11 days were transduced with either miR-142 or GFP lentivirus. On day 6 after transduction, cells were harvested and Western blot analysis was performed for MAOA. Representative Western blots from three human neuron donors indicating a decrease in MAOA protein level after miR-142 transduction compared to control GFP transduction are shown. Quantification of Western blots from 5 human neuron donors revealed a significant decrease in MAOA level after miR-142 transduction (fold change -1.9, p<.05). Blots were normalized to β-actin and paired t-test was performed.</p