Monad regulates cell invasion of MDA-MB-231 cells.

Abstract

<p>Invasiveness of GFP or Monad overxpressing MDA-MB-231 cells (A), tetracycline-regulated Monad overexpressing MDA-MB-231 cells (B), and control or Monad siRNA treated MDA-MB-231 cells (C) was assayed by a Boyden chamber method 2 days after transfection. In (B), Monad was overexpressed by doxycycline (Dox). Results are presented as means of the number of cells/well with S.E. Cells were cultured in triplicate wells/experiment and the experiment was replicated three times. (C) Knockdown of Monad increased invasiveness of MDA-MB-231 cells. Amphiregulin-neutralizing antibody (1 µg/ml) inhibited the increased invasion by Monad siRNA. *<i>P</i><0.01 vs. control. ^†<i>P</i><0.01 vs. control IgG.</p