<p>MDA-MB-231 cells were transfected with amphiregulin promoter (A) or 3′-UTR (B) -luciferase (Luc) reporters and after 2 days they were analyzed using luciferase reporter assays. The results represent the mean values with S.E. from three independent experiments. (C) MDA-MB-231cells were treated with either control or Monad siRNA and after 2 days with 5 µg/ml of actinomycin D (ActD). Relative amphiregulin mRNA levels at the different time points were analyzed by real-time RT-PCR and expressed as percentages of the level at the 0-h time point from four independent experiments (mean ± S.E.). Data were normalized based on GAPDH mRNA copy numbers. (D) The decay rates of amphiregulin were determined using the Click-iT Nascent RNA Capture Kit (Invitrogen) and expressed as percentages of the level at the 0-h time point from four independent experiments (mean ± S.E.). Data were normalized based on GAPDH mRNA copy numbers.</p
