Glycoprotein staining of transgenic lines and WT line in rice seeds.

Abstract

<p>Total protein was extracted from mature dehulled seeds of SB-57 and SBK-66 transgenic lines and WT. RNase B was used as the glycoprotein control. To evaluate endoglycosidase digestion efficiency, RNase B was digested by PNGase F and Endo H. Lane 1: marker; lane 2: WT; lane 3: SBK-66; lane 4: SK-57; lane 5: RNase B control without digestion; lane 6: RNase B digested by PNGase F; and lane 7: RNase B digested by Endo H. Band 1: PNGase F; band 2: RNase B; band 3: RNase B with Endo H digestion, and band 4: RNase B with PNGase F digestion.</p