Poly I:C induces production of IL-7 in human salivary gland epithelial cells.

Abstract

<p>(A) HSG cells were cultured <i>in </i><i>vitro</i> with or without poly I:C for 1 and 3 days. IL-7 mRNA levels, left panel, and protein concentrations in culture supernatants, right panel, assessed by real time PCR and ELISA, respectively. (B) HSG cells were treated with IFN-α, IFN-γ or both for 1 and 3 days, and then measured for IL-7 mRNA levels. (C) HSG cells were treated with poly I:C in the presence of control IgG or anti-human IL-7 for 1 or 3 days, and then measured for BAFF mRNA expression. All PCR results are presented relative to that of GAPDH. All data are the average of analyses of 6 independent samples for each group (3 samples per experiment, total 2 independent experiments). </p

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