<p>(<b>A</b>) Developmental Northern blots showing <i>loh</i> and <i>prc</i> expression in total RNA samples of 0–24 h old embryos (E), first, second or third instar larvae (L1–L3), mid-stage pupae (P) or adults (A) using gene specific riboprobes (indicated in B and C). (<b>B, C</b>) Schematic representation of <i>loh</i> (B) and <i>prc</i> (C) gene loci and transcripts. The schemes indicate the position of transposons, location of hairpins (IR) used for knock down and riboprobes used for Northern analysis (NB) and <i>in situ</i> hybridization (ISH). (<b>D</b>) Immunoblot of total protein extracts obtained from stage 17 control, homozygous Df(2L)Exel7048 or homozygous <i>loh<sup>MB05750</sup></i> embryos probed with antibodies against Loh or βTub. Loh is undetectable in homozygous deficiency or mutant extracts. (<b>E</b>) Immunoblot of total protein extracts obtained from control or homozygous <i>prc<sup>MB03017</sup></i> 0–24 h old embryos (E), third instar larvae (L3) mid-stage pupae (P) or adults (A) probed with antibodies against Prc or βTub. Prc is undetectable in extracts of homozygous mutants.</p
