Loh induces Prc matrix formation <i>in vivo</i>.

Abstract

<p>(<b>A</b>) Prc (green) is absent from the ECM of larval muscles or the fat body in wild type third instar larvae. (<b>B</b>) Schematic representation of ectopically expressed LohA wild type protein or the secretion defective variant LohA^ΔSP. (<b>C</b>) Expression of LohA (red) in adipocytes or myocytes results in the formation of a dense Prc matrix (green) along both cell types in third instar larvae, while secretion of LohA is essential to mediate Prc recruitment. Inset shows accumulation of LohA at muscle attachment sites. (<b>D</b>) Loh and Prc partially co-localize in an artificial matrix around adipocytes. Loh distributes as weak fibers accumulating in a spotted fashion along cellular contacts (arrowheads). (<b>E</b>) Single optical slice of adipocytes. Loh co-localizes to Prc (arrowhead) at the root of Prc fibers (double arrowhead). (<b>F</b>) Loh co-immunoprecipitates with Prc and vice versa from total adult protein extracts demonstrating a biochemical interaction of both proteins.</p