Purification of the PEGylated Sak by ald5k and ald20k.

Abstract

<p>(A) The sample was loaded on an SP Sepharose HP column (16 mm ×25 mm). The column was equilibrated with 12 ml of 50 mM NaAc–HAc buffer (pH 5.0, Buffer A) and then with 18 ml 0.5 M NaCl in Buffer A at a flow rate of 1.0 ml/min. (B) The fractions corresponding to the proteins were loaded on a Superdex 200 column (2.6 cm ×60 cm) equilibrated and eluted with 20 mM sodium phosphate buffer (pH 7.2) at a flow rate of 3.0 ml/min.</p