High glucose treatment increases C99 and Aβ₄₀ production.

Abstract

<p>The 20E2 cells were cultured and treated with different concentrations of glucose for 24 hours. Media containing 5.5 mM glucose served as control. The cell lysates were analyzed by western blot (<b>A</b>).C99 was detected by C20 antibody. β-actin, serving as internal control, was detected by AC-15 antibody. Quantification of C99 after 24-hour treatment of high glucose in 20E2 cells (<b>B</b>) The values are expressed as mean±S.E.M, n = 3,*p<0.001 by ANOVA The level of Aβ₄₀ in conditioned media of 20E2 cells was measured by ELISA(<b>C</b>). The values are expressed as mean±S.E.M, n = 4, *p<0.001, by ANOVA.</p