High glucose treatment increases full-length APP protein level.

Abstract

<p>SH-SY5Y cells were treated with different concentration of glucose for 24 hours (<b>A</b>), and 48 hours(<b>C</b>) with2.5 mM glucose treatment serving as control. The cell lysates were analyzed by Western blot. Full-length APP was detected by C20 antibody. β-actin, serving as internal control, was detected by AC-15 antibody. 24-hour and 48-hour of high glucose treatment significantly increased full-length APP protein level.Quantification of full-length APP after 24-hour treatment of high glucose in SH-SY5Y cells (<b>B</b>). The values are expressed as mean±S.E.M, n = 4,*p<0.05 by ANOVA. Quantification of full-length APP after 48-hour treatment of high glucose in SH-SY5Y cells (<b>D</b>) The values are expressed as mean±S.E.M, n = 3,*p<0.05 by ANOVA.</p