High glucose treatment does not affect APP transcription.

Abstract

<p>The 2.94 kb human APP promoter was transfected into SH-SY5Ycells and treated with high glucose for 24 hours (<b>A</b>) and 48 hours (<b>B</b>). 2.5 mM glucose serves as control. Luciferase assasy was performed.High glucose treatment did not affect APP promoter activity. All the promoter data shown are results of 4 independent experiments, with each condition performed in triplicates. The values are expressed as mean±S.E.M. n = 4, by ANOVA. SH-SY5Y cells were treated with different concentration of glucose for 24 hours(<b>C</b>) and 48 hours (<b>E</b>). RNA was extracted and APP mRNA level was measured by semi-quantitative PCR with specific primers. β-actin served as an internal control. 24-hourand 48-hourtreatment of high glucose did not significantly affect APP mRNA. Quantification of full-length APP after 24-hour treatment of high glucose (<b>D</b>)The values are expressed as mean±S.E.M, n = 7, by ANOVA. Quantification of full-length APP after 48-hour treatment of high glucose (<b>F</b>)The values are expressed as mean±S.E.M, n = 5, by ANOVA.</p