Pairwise interactions among Pil proteins in Y2H system.
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Abstract
<p>Panels <b>A</b> and <b>B</b>. Interactions among <i>M. xanthus</i> Pil proteins. Panels <b>C, D</b> and <b>E</b>. Interactions among <i>T. thermophilus</i> Pil proteins. The first and second columns on the left of each panel indicate Pil proteins or fragments fused to GAD and GBD in Y2H plasmids by their last letter, respectively. V indicates an empty Y2H vector. N0, N1, N2 and their combinations are represented by their numerals only. Last row in each panel contained the positive control with T-antigen (T) and p53 (53). The left half of each panel shows growth on SD-His and the right on SD-Ade plates, respectively. The spots in each row in a panel were inoculated by serial dilutions of the same yeast cells with the indicated Y2H plasmids. See text and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070144#s2" target="_blank">Materials and Methods</a> for details.</p