<i>pilB^WA</i> suppresses some but not all T4P⁻<i>pil</i> deletions in EPS production.

Abstract

<p>EPS levels were analyzed on plates with the fluorescent dye Calcofluor white (See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070144#s2" target="_blank">Materials and Methods</a>). The presence of fluorescence indicates EPS⁺ and its absence EPS⁻. Each strip shows two strains, the upper is a strain with the indicated mutation(s) in a <i>pilB⁺</i> background and the lower in the <i>pilB^WA</i> background. Strains used are YZ690 (Δ<i>pilA</i>), YZ1573 (Δ<i>pilA pilB^WA</i>), DK10417 (Δ<i>pilC</i>), YZ1182 (Δ<i>pilC pilB^WA</i>), YZ1860 (Δ<i>pilM</i>), YZ1185 (Δ<i>pilM pilB^WA</i>), DK11135 (Δ<i>pilG</i>), YZ1183 (Δ<i>pilG pilB^WA</i>), DK11133 (Δ<i>pilH</i>), YZ1181 (Δ<i>pilH pilB^WA</i>), DK11132 (Δ<i>pilI</i>), YZ1184 (Δ<i>pilI pilB^WA</i>), YZ1191 (Δ<i>pilA</i> Δ<i>pilQ</i>) and YZ1190 (Δ<i>pilA</i> Δ<i>pilQ pilB^WA</i>). <i>pilB^WA</i> failed to suppress Δ<i>pilN</i>, Δ<i>pilO</i>, Δ<i>pilP</i> and Δ<i>pilQ</i> as it did Δ<i>pilC</i> and Δ<i>pilM</i> (data not shown).</p