Oligomeric interface affects DNA binding and cooperativity.

Abstract

<p>(A) Dissociation constants for mutants of LANA<sub>DBD</sub> as determined by fluorescence polarization using an LBS1 oligomer. (B) Representative isotherms of the FP assays for wild-type (cooperative), F1037A/F1041A (non-cooperative), 1021–1153 (increased cooperativity), and M1117A (negative cooperativity) to illustrate the fit of the data using a Hill coefficient. The error bars represent the standard deviation of three independent replicates. Fits not shown in this panel are available in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003672#ppat.1003672.s003" target="_blank">Figure S3</a>.</p

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