<p><b>A)</b> Representative immunofluorescence confocal microscopic images of the F-actin (red) of cells on substrate with beads inside. <b>B)</b> Representative immunofluorescence confocal microscopic images of the F-actin (red) of cells on substrate with beads on surface. <b>C)</b> Statistical quantification of the mean fluorescence intensity of actin within the HeLa cells on substrate with different positioned beads (n = 28 for beads inside, n = 34 for beads on surface). <b>D)</b> Representative immunofluorescence confocal microscopic images of the vinculin (green) with beads inside. <b>E)</b> Representative immunofluorescence confocal microscopic images of the vinculin (green) with beads on surface. <b>F)</b> Comparison of total vinculin area on PAA gels with beads inside and beads on the gel surface (n = 22 for the former, n = 20 for the latter). Bars represent mean ± standard deviation. Two-tailed t-test was performed for statistical analysis in both <b>C)</b> and <b>F)</b>.</p
