<p>(A) Total RNA was obtained from central marrow ECs transduced with shRNA clones targeting PlGF. The mRNA expression level of PlGF was measured using quantitative RT-PCR and normalized to <i>hprt</i> levels (*p<0.05, ***p<0.001; n = 6 from 3 independent experiments; error bars represent standard deviation). (B) Uptake of DiI-Ac-LDL (red) in control-transduced and PlGF knockdown central marrow ECs. Nuclei were visualized with DAPI (Scale bar: 50 µm). (C) The formation of endothelial capillary like tube was evaluated under a phase-contrast microscope on Matrigel following 10 hours of culture (Scale bar: 200 μm). (D, E) LSK cells were seeded in serial dilutions on central marrow ECs, central marrow ECs transduced with E8, E12 or E8 and E12 lentiviral shRNA-PlGF vectors or shRNA-GFP vector and cultured at 33°C and 5% CO<sub>2</sub>. CAFCs were scored on week 2 and 5 (*p<0.05, **p<0.01; n = 3 from 3 independent experiments; error bars represent standard deviation).</p
