Larval antenna is a peripheral thermosensory organ.

Abstract

<p><b>a</b>) Arithmetic means ± S.E.M of total distance travelled in 300s for individual larva recorded from larvae lacking either antennae or maxillary palp were plotted (n≥12). Asterisks suggest <i>p</i><0.05 using Mann–Whitney <i>U</i> test to compare antennal ablation to sham ablation treatment. Kruskal-Wallis one-way analysis of variance was also utilized to compare larval mobility at all 5 temperatures following antennal ablation with <i>p</i>>0.05, indicating larvae without antennae were not capable of eliciting differential mobility at varying ambient temperatures comparing to sham treatment. <b>b</b>–<b>k</b>) Localization of AgTRPA1 mRNA was detected by fluorescent in situ hybridization (FISH). White arrow indicates localization of AgTRPA1 mRNA while green labels neuronal axons and dendrites. <b>l</b>–<b>o</b>) Red fluorescence indicates AgTRPA1 mRNA while green indicates the localization of AgOrco protein that is expressed in all ORNs. White arrow indicates AgTRPA1-expressing neuronal cell bodies while hollow arrow shows cluster of ORNs (Scale bar, 25µm).</p