Knockdown of AgTRPA1 mRNA via RNAi.
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Abstract
<p>Means of cycle threshold (CT) values for amplification of <i>agtrpa1</i> and <i>rps7</i> were shown (n=2). Quantitative RT-PCR was performed on cDNA isolated from whole larvae receiving AgTRPA1, non-specific siRNA and buffer injection. Relative mRNA abundance + S.E.M was plotted with data normalized to non-specific siRNA treatment using PFAFFL method.</p