YAP and TAZ are phosphorylated by Lats2 and accumulate in the cytoplasm.

Abstract

<p>(A) Lats2-mediated enhanced phosphorylation of YAP and TAZ. Whole-cell lysates were prepared from Lats2-transfected cells, immunoblotted with Lats2, p-YAP, YAP, p-TAZ, TAZ and Tubulin antibodies. (B) Lats2-mediated enhanced cytoplasmic accumulation of YAP and TAZ. Micrographs depict YAP and TAZ in 3T3L1 cells, as detected by anti-YAP and anti-TAZ antibodies (green). The nucleus was stained by DAPI (blue). The scale bar represents 20 µm.</p