The absence of MCP-1 reduced fat mass and improved metabolic perturbation induced by OVX.

Abstract

<p>WT mice (open bar) and MCP-1-KO (oblique-lined bar) mice were subjected to OVX or sham surgery, and then held for 12 weeks. Whole body weight change up to 12 weeks after surgery (A) and average daily food intake (B) were measured. *, <i>P</i><0.05; WT OVX vs. MCP-1-KO OVX mice. Adipocyte volume was calculated from photograph of hematoxylin-eosin staining of visceral fat, assuming that an adipocyte is a sphere (magnification, ×200). Scale bar, 100 µm (C). Glucose clearance (D) and insulin sensitivity (E) were determined 12 weeks after sham or OVX, following an intraperitoneal injection of glucose (1 mg/kg) and insulin (0.75 munits/kg), respectively. AUC was measured for each group for D and E. *, <i>P</i><0.05, ***, <i>P</i><0.001; WT OVX vs. MCP-1-KO OVX. Data are expressed as mean ± SEM. Differences between groups were analyzed by two-way ANOVA, followed by Bonferroni post-tests (C, D) (adipocyte volume; <i>P</i><0.001, AUC for glucose tolerance; <i>P</i><0.01, effect of surgery. adipocyte volume; <i>P</i><0.001, AUC for glucose tolerance; <i>P</i><0.05, effect of MCP-1). *, <i>P</i><0.05; ***, <i>P</i><0.001 compared with WT OVX mice. Similar results were obtained in three independent experiments.</p

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