Subcellular localizations of AoSO-EGFP and AoPab1-mDsRed in response to heat stress.

Abstract

<p>(A) A wild-type strain co-expressing AoSO-EGFP and AoPab1-mDsRed was used to examine the relative localizations of AoSO and stress granules under normal growth conditions and heat stress. Approximately 10<sup>4</sup> conidia of cells were grown in CD medium at 30°C for 18 h before being exposed to 45°C for 10 min. Arrowheads indicate AoSO foci, and arrows indicate stress granules. Colocalization of a stress granule and an AoSO cytoplasmic focus is indicated by the asterisk. The effect of cycloheximide on the formation of mRNP granules was examined by pre-treating cells with 200 µg/ml cycloheximide for 30 min before being exposed to heat stress. Scale bar  =  5 µm. (B) Effect of cycloheximide on the formation of AoSO cytoplasmic foci at the hyphal tip. Cells were incubated with CD medium containing 200 µg/ml cycloheximide for 30 min before being exposed to heat stress. The percentage of cells displaying AoSO cytoplasmic foci at the hyphal tip was determined. Error bars represent the standard error. ***P < 0.0001. The presented data are from three independent experiments, each with n  =  50.</p

    Similar works

    Full text

    thumbnail-image

    Available Versions