<i>Aopub1</i> disruptant showed defects in the conidia formation and more severe growth retardation in stress conditions.

Abstract

<p>(A) Stress-induced formation of stress granules (indicated by arrows) in an AoPub1-EGFP expressing wild-type strain. Approximately 10<sup>4</sup> conidia of cells were grown in CD+Met medium at 30°C for 18 h before being exposed to ER stress (10 mM DTT, 60 min), oxidative stress (2 mM H<sub>2</sub>O<sub>2</sub>, 30 min), and osmotic stress (1.2 M sorbitol, 30 min). Scale bar  =  5 µm. (B) Approximately 10<sup>3</sup> conidia of wild-type and <i>Aopub1</i> disruptant cells were spotted onto PD plates, and cultured at 30°C for 4 days. (C) Approximately 10<sup>3</sup> conidia of wild-type and <i>Aopub1</i> disruptant cells were spotted onto PD plates with or without 10 mM DTT, 2 mM H<sub>2</sub>O<sub>2</sub>, or 1.2 M sorbitol, and cultured at 30°C for 4 days. Colony diameters were compared to those of the wild-type strain under the control condition, which was set to 100%. Data represent the mean ± S.D. of three biological replicates. The asterisk denotes a statistically significant difference, as judged by the Student’s t-test with P < 0.005 (**) and P < 0.0005 (***).</p

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