<p>(<i>A</i>) Amino acid sequences of membrane-tethered peptides. (<i>B</i>, <i>C</i>) Yeast strain IMFD-72ZsD, which coexpresses pGK421-NTSR1 and either pGK426-NTS42 (NTS), pGK426-NTS(8-13)42 (NTS(8-13)), pGK426-NMN42 (NMN) or pGK426-alpha42 (α-factor), was incubated in pH-adjusted SD selective medium. (<i>B</i>) The GFP fluorescence of 10,000 cells was measured by flow cytometry. Mean values of the green fluorescence signal of 10,000 cells. Error bars represent the standard deviations (<i>n</i> = 3); *, <i>p</i> < 0.05, and ***, <i>p</i> < 0.001, by one-way ANOVA, Tukey’s post test. (<i>C</i>) Fluorescence microscopy analysis of the cells incubated for 24 h. Cells were examined using the 40× objective lens of a fluorescence microscope. Exposure time was 0.67 s. The left photographs are fluorescence micrographs, and the right photographs are bright-field micrographs. </p
