<p><b>A</b>. Screening for interactors of XopN<sub>KXO85</sub> in rice using a yeast two-hybrid system. S (strong: pEXP <sup>TM</sup>32/Krev1 + pEXP <sup>TM</sup>22/RalGDS-wt), W (weak: pEXP <sup>TM</sup>32/Krev1 + pEXP <sup>TM</sup>22/RalGDS-m1), and A (absent: pEXP <sup>TM</sup>32/Krev1 + pEXP <sup>TM</sup>22/RalGDS-m2) indicate the strength of each interaction. Three independent and representative colonies are shown for each bait–prey combination. <b>B</b>. <i>In vivo</i> pull-down analysis of XopN<sub>KXO85</sub> and OsVOZ2 (left panel) and XopN<sub>KXO85</sub> and OsXNP (right panel). Total proteins from <i>N</i><i>. benthamiana</i> leaves co-expressing XopN<sub>KXO85</sub>-6× His and Flag-OsVOZ2 or XopN<sub>KXO85</sub>-6× His and OsXNP-Flag protein were purified by Ni<sup>+</sup> affinity chromatography followed by Western blotting using anti-His and anti-Flag antibodies. The expected molecular weights were as follows: XopN<sub>KXO85</sub>-6× His = 78.7 kDa; Flag-OsVOZ2 = 74.6 kDa; OsXNP-Flag = 40.1 kDa; +, protein expressed; and -, vector control. <b>C</b>. BiFC analysis of XopN<sub>KXO85</sub> -OsVOZ2, XopN<sub>KXO85</sub> -OsXNP, and XopN<sub>KXO85</sub> -OsVOZ1 interactions in <i>N</i><i>. benthamiana</i> leaves. Negative, pDEST-SCYNE(R)<sup>GW</sup> + pDEST-SCYCE(R)<sup>GW</sup>; positive, pEXP-SCYNE(R)-Cnx7 + pEXP-SCYCE(R)-Cnx6. Bars = 50 µm.</p
