<p>(A) Bisulfite sequencing result of the <i>ζ-η</i> locus reveals significant methylation in all DNA clones examined. Each row of circles represents the number of cytosines in one subcloned <i>ζ-η</i> fragment. Opened and filled circles indicate unmethylated and methylated cytosines, respectively. (B) Strategy for detecting methylation in <i>disi-6</i> locus (strategy 1, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003761#s4" target="_blank">Materials and Methods</a>). The lack of cleavage by <i>Dpn</i>II indicates no methylation at the GATC recognition site of the original sequence, whereas the cleavage by <i>Dpn</i>II indicates that the DNA sequence contained 5mC. (C) 9 clones from “cut” and “uncut” populations were subject to sequencing. DNA methylation profiles were shown. (D) Southern blot analyses of <i>disi-29</i> and <i>disi-47</i> loci. The <i>am</i> and <i>ζ-η</i> loci were as the negative and positive control, respectively.</p
