<p>A-F: A1847, A2780, and OVCAR10 were treated with 20 µg/mL epimorphin for 3 days. After 3 days, epimorphin-treated and untreated OCCs were cultured in triplicate with serial doses of carboplatin for an additional 3 days. Cell viability was quantified using a CellTiter Blue® assay (A–C). Apoptosis was quantitated using a Guava Nexin assay (D–F). A–C: IC<sub>50</sub> values indicate carboplatin induced more cell viability loss in all three epimophin-treated OCCs than the untreated controls in a dose-dependent manner. D–F: Detection of apoptotic responses was found to increase in all three epimorphin-treated OCCs with increasing concentrations of cisplatin compared to those of the untreated controls. Data were normalized to the controls and are represented as means ± S.D. [*<i>p</i><0.05 compared with control]. Images of apoptotic cells were captured at 10X magnification, with at least 3 images per well, using an upright phase-contrast microscope (T3.15A; Fisher Scientific).</p
