Expression of Hif-1α apparently increased in liver tissues of <i>Schistosoma</i><i>japonicum</i> infected mice.

Abstract

<p>BALB/c female mice, 6–8 weeks old, were percutaneously infected with 25 cercariae of <i>Schistosoma</i><i>japonicum</i> through the shaved abdomen, sacrificed at 6 weeks post-infection and samples of liver were collected. The expression of Hif-1α and α-SMA in <i>Schistosoma</i><i>japonicum</i> infected (n=3) and non-infected (n=3) mice liver was detected with western blot and immunohistochemistry. (A) 1: protein extract from the liver of non-infected Balb/C mice, 2: protein extract from livers of <i>Schistosoma</i><i>japonicum</i> cercariae infected Balb/C mice. (B) Densitometric analysis (n=3). Data are mean ± SD. * : <i>P</i><0.05, ** : <i>P</i><0.01. (C) Acute inflammatory cell infiltration and granulomatous inflammation, surrounding eggs of <i>Schistosoma</i><i>japonicum</i>. (D) Expression of Hif-1α in non-infected mice liver. (E) Expression of Hif-1α in infected liver.</p