1% O₂ induced apparent accumulation of Hif-1α and reorganization of F-actin in HSC-T6 cells.

Abstract

<p>HSC-T6 cells, rat hepatic stellate cell line, were cultured in room air or in 1% oxygen. (A) Cells were collected at indicated time and cell lysates were subjected to detect Hif-1α and β-actin with western blot. (B) F-actin was stained using Fluorescein Isothiocyanate (FITC)-labeled phalloidin to capture F-actin of cells grown on the coverslips.</p