<p>Western blotting detects the 43-D stripped from polypropylene tubes using Laemmli Buffer (A). 1 through 6 denotes the tube order during serial transfer. The amount of SP-D detected on uncoated tubes was quantified using ImageJ software (B). Columns with an asterisk (*) are significantly different (p≤0.05), as are columns labeled with the number sign (#, p≤0.01).</p