Effect of gestational zinc deficiency on T cell proliferation.

Abstract

<p>Splenocytes from each group were obtained on day 14 after the third HBV vaccination and analyzed for the HBsAg-specific T cell proliferative response, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073461#s2" target="_blank">Materials and Methods</a> and expressed as SI. The final concentration in the 96-well plate for ConA, BSA and recombinant HBsAg was 2.5, 30 and 20 µg/ml, respectively. The data are expressed as mean ± S.D. of 3 animals in the proliferative assay from one representative experiment. Each experiment was performed 3 times. ZD: pups from the zinc deficiency group; ZS: pups from the zinc supplemented group.</p