Immunoreactive IL-13RA2 in human and canine brain tumor specimens/cells by purified MAb’s of Peptide 1

Abstract

<p><b><i>A</i></b>, Human glioblastoma (G), <b><i>B</i></b>, oligodendroglioma (O), astrocytoma (A), normal brain (NB), and G14 human GBM tumor lysate; meningioma (M), <b><i>C</i></b>, tissue lysates immunoreactivity using Western blots. Canine astrocytoma, glioblastoma and normal brain, <b><i>D</i></b>; oligodendroglioma, gliosarcoma (GSO) and mixed astro-oligo (AO), <b><i>E</i></b>; and choroid plexus papilloma (CPP) and meningioma, <b><i>F</i></b> tissue lysates immunoreactivity using western blots. Western blot of cell lines and parent tumor tissue obtained from dogs with spontaneous GBM, <b><i>G</i></b>. Immunoprecipitation of IL-13RA2 from U-251 MG cell lysate using either MAb 2G12C3, MAb 2G12E2 or a polyclonal antibody (R&D Systems #AF146); the polyclonal antibody was used for the receptor detection after immunoprecipitation<b><i>, H</i></b>.</p

    Similar works

    Full text

    thumbnail-image