Imunoreactivity of MAbs obtained with immunization using Peptide 3.

Abstract

<p>Reactivity of MAbs raised against Peptide 3 in ELISA using recombinant IL-13RA2-Fc, <b><i>A</i></b>; and the synthetic Peptide 3, <b><i>B</i></b>. Detection of IL-13RA2, but not of IL-13RA1-Fc with MAbs 1E10B9 and 1E10F9, <b><i>C</i></b>. Immunoreactive IL-13RA2 in Western blot of U-251 MG and T98G cell lysates using MAb 1E10B9, <b><i>D</i></b>. Expression of IL-13RA2 detected by immunohistochemistry using MAb 1E10B9 in human GBM specimens and normal brain, <b><i>E</i></b>, and canine GBMs and normal brain, <b><i>F</i></b>.</p