Modes of motility of

Abstract

<div><p><i>T</i><i>. annulata</i>-<b>infected </b><b>macrophages </b><b>embed </b><b>in </b><b>matrigel</b>. </p> <p>(A) Motility of cells embedded in collagen, matrigel or Qgel matrices was recorded for 15 h by live-video microscopy. Vertical scatter dot plots show speed calculated as µm/min (collagen: 55 cells, matrigel: 19 cells, QGel: 10 cells). T-test, *** p < 0.0001. B) Infected cells adopt different modes of motility in matrigel. These include forward movement by tunneling, which requires matrix degradation/engulfment at the leading edge (movie S3 and S5). Alternatively, cells can move in a saltatory mode, where cells squeeze through narrow pores and forward movement oscillates between rapid movements and phases of stagnation (movie S4 and S6). Vertical scatter dot plots show percentage of cells migrating in tunneling (14%) or saltatory (38%) mode. 48% of the cells could not be allocated to one of the two modes (immobile). 147 cells total, T-test, ** p = 0.0014. C) X/Y plots show representative paths of cells migrating in the tunneling (left) or in the saltatory mode (right). Dot plots show representative track lengths of tunneling and saltatory migration in µm. Speed diagrams compare fluctuations in speed development of tunneling and saltatory movements; peak = maximum speed, valley = minimal speed or stagnation.</p></div