Membrane blebs evolve along filopodia-like protrusions at leading edge.

Abstract

<p><b>A</b>) Still images of DIC live-cell microscopy (movie S14). Arrowheads indicate filopodia-like protrusions, arrow neck zone of maximal compression. <b>B</b>) Higher magnification of leading edge of cell shown in A. Arrowhead highlights blebbing protrusion at maximal expansion. <b>C</b>) Quantification of surface area of blebbing protrusion shown in B. <b>D</b>) Confocal microscopy image of TaH128101 cells invading matrigel. Green: F-actin, red: parasite surface (anti-TaSP), blue: DNA. Insets show leading F-actin and host cell nuclear DNA. <b>E</b>) as D but cells were incubated in the presence of either the Src kinase inhibitor PP2 (5 µM) or the Rho kinase inhibitor H-1152 (5 µM). <b>F</b>) model of F-actin distribution and leading edge membrane dynamics of TaH12810 cells invading matrigel.</p