TuMV 6K<sub>2</sub>-tagged vesicles do not colocalize with endocytic markers.
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Abstract
<p>Three-dimensional rendering of 22 1 µm thick confocal images that overlap by 0.5 µm of <i>N. benthamiana</i> agroinfiltrated leaves. (A) shows the distribution of TuMV-induced 6K<sub>2</sub>:mCherry-tagged structures and YFP labeled Ara7 dots. Left panels; red fluorescence channel imaging TuMV producing 6K<sub>2</sub>:mCherry, middle panel; green fluorescence channel imaging YFP-RabF2b, and right panel; merged images. (B) shows the distribution of TuMV-induced 6K<sub>2</sub>:GFP-tagged structures and FM4-64 labeled vesicles. Left panel shows green fluorescence channel from TuMV producing 6K<sub>2</sub>:GFP, middle panel red fluorescence channel from FM4-64, and right panel merged images. Images were taken at 4 dpinf. Scale bar = 20 µm.</p
