Knockout of <i>Vps35 in vivo</i>.

Abstract

<p>(A) PCR analysis using primers that anneal outside <i>Vps35</i> exon 4 shows deletion of exon 4 from genomic DNA isolated from small intestinal epithelium (SI) of 4-OHT induced <i>Vps35</i>^<i>fl/fl</i> (control) or <i>Vps35</i>^<i>fl/fl</i><i>; Villin-CreERT2</i> mice (<i>Vps35^∆/∆</i>). Histological analysis of <i>Vps35</i> knockout intestine showed no defects in crypt-villus morphology. Intestine sections were Periodic Acid Schiff (PAS) stained (B) and immunohistochemistry was performed to stain Lysozyme (C). </p