Abstract

<p><b>A.</b> Workflow of the SERPA process including 2DE-gel separation of lysates from either hypoxic or normoxic tumor cells, membrane transfer, immunoblotting with the serum from either control or tumor-bearing mice, and detection of spots of interest. <b>B.</b> Typical immunoblotting patterns resulting from the incubation of 2D-resolved lysates of HCT116 cells exposed to normoxia or hypoxia, with the indicated mouse serum. In the bottom panels, proteins of the lysates are labelled with Cy dye (red) and fixed antibodies are detected with an anti-mouse secondary antibody (green spot); arrow indicates the presence of a protein exclusively detected in the lysates of hypoxic tumor cells by antibodies from the serum of tumor-bearing mice.</p

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