The mushroom body is critical for the DopEcR-dependent processing of courtship memory.

Abstract

<p>(A) Rescue of the <i>DopEcR<sup>PB1</sup></i> memory defect by expression of wild-type <i>DopEcR</i> transgene using the <i>DopEcR</i>-Gal4 driver. (B) Rescue of <i>DopEcR<sup>PB1</sup></i> memory defect by expression of wild-type <i>DopEcR</i> transgene under control of the MB-specific c772, c739 and 201 y drivers. Note that MB-Gal4 lines drive reporter expression in different subsets of MB neurons (see text). (C) Courtship memory defect induced by MB-specific expression of the <i>DopEcR</i> RNAi using the c772 and c739 drivers. The presence or absence of courtship memory was evaluated by applying Mann–Whitney U-test to naïve and conditioned flies. Statistical significance is shown above each bar. NS, no significant difference. **, <i>P</i><0.01; ***, <i>P</i><0.001. Sample numbers for naïve and conditioned flies are shown under each graph. PIs were analyzed using Student's t-test or Krustal-Wallis One-Way ANOVA, followed by Dunn's pairwise test for multiple comparisons. #, <i>P</i><0.05; ###, <i>P</i><0.001. Error bars (s.e.m.).</p

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