<i>N. crassa</i> SIN components are required for septum formation but display distinct mutant characteristics

Abstract

<p>(<b>A</b>) Deletion strains defective in the indicated SIN components generate thin and aseptate hypha in young colonies (18 h time point). In older colonies, the septation defects were suppressed in ∆<i>sid-1</i> and ∆<i>cdc-14</i> strains (36 h time point). Cell wall and septa were labeled with Calcofluor White. (<b>B</b>) SIN mutants showed cytoplasmic leakage (magnified inserts), but, due to the fast ability to septate, ∆<i>sid-1</i> and ∆<i>cdc-14</i> generated abundant aerial mycelium and asexual spores (conidia; plate morphology). (<b>C</b>) SIN mutants displayed distinct abnormalities during sexual development. wt x ∆ crosses with ∆<i>cdc-7</i>(het) and ∆<i>dbf-2</i>(het) resulted in the frequent formation of large, banana-shaped ascospores. In contrast, wt x ∆<i>sid-1</i>(het) progeny morphology was normal, while crosses of wt x ∆<i>cdc-14</i>(het) produced no mature perithecia .</p