Evaluation of protein expression (Western-blot).

Abstract

<p>(<b>A</b>) Protein ratios were calculated relative to β-actin (loading control). <b>§</b>: <i>P</i><0.01 compared with SHO group, <b></b>: <i>P</i><0.01 compared with UUO group, <b>☆</b>: <i>P</i>>0.05 compared with UUO group. (<b>B</b>) Representative Western blot for PHB, TGF-β1, Col-I, Col-III, Col-IV, and FN in SHO (sham operation), UUO (model group subjected to unilateral ureteral obstruction), PHB⁻ (UUO rats treated with lentivirus carrying siRNA PHB), and PHB⁺ (UUO rats treated with lentivirus carrying PHB) groups; Negative control: UUO rats treated with control viruses.</p