Analysis of rhG-CSF protein by SDS-PAGE.

Abstract

<p>Upon IPTG induction, rhG-CSF was analyzed using a 4–12% reducing SDS-PAGE gel followed by Coomassie brilliant blue staining. <b>A.</b> Lane 1, cell homogenates of <i>E. coli</i> JM109/pPT-G-CSF without IPTG induction; lane 2, cell homogenates after IPTG induction for 1 h; lane 3, After IPTG induction for 3 h; lane 4, After IPTG induction for 5 h. <b>B.</b> Lane 1, total homogenates; lane 2, supernatant after centrifugation; lane 3, Pellet after centrifugation. Most of IPTG induced rhG-CSF is pelleted after centrifugation. The arrow indicates rhG-CSF.</p