The analysis of rhG-CSF protein by SDS-PAGE and IEF.

Abstract

<p><b>A</b>. A 4–12% discontinuous NuPAGE SDS-PAGE gel and Coomassie brilliant blue staining were used to confirm the purity of rhG-CSF. Lane 1, molecular weight marker; lane 2, standard rhG-CSF (Filgrastim); lane 3, rhG-CSF. <b>B</b>. The Novex pH 3–10 IEF gel was used to examine the purity of rhG-CSF. The IEF marker indicates pI. Lane 1, pI marker 4.5–7.4; lane 2, standard hG-CSF; lane 3, purified rhG-CSF. The arrow indicates rhG-CSF.</p