Prep-HPLC chromatogram and IEF analysis.

Abstract

<p>Prep-HPLC chromatography was performed to detect rhG-CSF protein. <b>A</b>. Chromatogram of prep-HPLC with the sup after pH precipitation of refolded rhG-CSF. <b>B</b>. IEF analysis of each fraction from A to H obtained by Prep-HPLC. The IEF marker indicates pI. Lane 1, pI marker 4.5–7.4. Absorbance is in milliabsorbance units (mAU).</p