Rosiglitazone inhibits NF-κB binding in IL-8/MCP-1 promoters.

Abstract

<p>HK-2 cells were treated without or with 10 µM RGL for 2 hours and then stimulation with 1 μg/ml LPS for 60 minutes. The representative agarose gel show PCR products of DNA obtained after chromatin immunoprecipitation with anti-p65 antibody (IP:anti-p65), non -immunoprecipitated DNA (input), or normal mouse IgG (negative control) amplified with primers specific for NF-κB site in IL-8/MCP-1 promoter. </p