Digestion of human haemoglobin by the recombinant human scabies mite aspartic protease N23SsAP.

Abstract

<p>The haemoglobinolytic activity of N23SsAP was detected using native human haemoglobin as substrate at pH% non-reducing SDS-PAGE gel and stained with coomassie brilliant blue R-250. Lane 1, molecular weight marker; lane 2, haemoglobin alone; lanes 3–6, haemoglobin plus N23SsAP, incubated for 1 hour (lane 3), 2 hours (lane 4), 4 hours (lane 5) or 12 hours (lane 6).</p