Assay of Behavioral Responses to Mandibular Gland Extracts.

Abstract

<p>Caged groups of 30 five-day-old workers were presented queen mandibular gland extracts from two different queen groups. The number of workers in each cage antennating and/or licking the two extracts were counted every five minutes over a 40-minute period and for two consecutive days. Statistical differences in the preferences for one extract versus the other in the pairwise comparisons were determined via repeated measures ANOVA with treatment as the main effect and day as a repeated variable. The mean ± SE is shown for the raw data, an asterisk above the bars indicates statistically significant differences; n is the number of cages used in comparison. <b>A</b>) In the first experiment we tested workers' preference for extracts of Virg, CO<sub>2</sub>, and CPM queens. <b>B</b>) In the second experiment we tested workers' preference for extracts of Virg, SA1, SA8, SE1, and SE8 queens.</p

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