Immunofluorescent staining and quantification of ID-related proteins of the EHTs cultured for 3, 7 and 14 days.

Abstract

<p>(<b>A–D</b>) At day 3, the cell membranes were positively stained with N-cadherin, Cx43, PKP2 and PG. (<b>E–H</b>) At day 7, N-cadherin (<b>E</b>), PKP2 (<b>G</b>) and PG (<b>H</b>) staining were positive and mainly localized to the contact sites of adjacent cells (arrow). Whereas the Cx43 positive staining was distributed along the whole plasma membrane in a punctuate pattern (<b>F</b>). (<b>I–L</b>) At day 14, ID-related proteins concentrated at the polar ends (arrow) of cardiomyocytes. (<b>M</b>) The FI of N-Cadherin, PKP2 and PG decreased from day 3 to 7 but increased at day 14. Cx43 was expressed the opposite. **<i>p</i><0.01; *<i>p</i><0.05.</p

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